A muscular issue forced the France international off after just 10 minutes of the Premier League meeting at Stamford Bridge, with Ruben Loftus-Cheek taking his place.
Maurizio Sarri may have to plan for the visit of Eintracht on Thursday without Kante, who completed the full 90 minutes in the 1-1 first-leg draw.
Kante suffered an injury to his ribs against Burnley on April 22 but was able to play a full part in Chelsea’s next two games.
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